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Infectious Canine Hepatitis(ICH) Test

Infectious canine hepatitis (ICH) due to canine adenovirus 1 (CAV-1) is a worldwide, infectious disease of dogs, with clinical signs that vary from a slight fever and congestion of the mucous membranes to severe depression, marked leukopenia, and coagulation disorders. Clinical signs and laboratory abnormalities often relate to the tendency of the virus to cause hepatocellular necrosis and diffuse endothelial cell damage; however, virus-associated immune complex deposition can also lead to uveitis, corneal clouding, and glomerulonephritis. Diagnosis can be made by the use of ELISA, serologic, or PCR testing, and postmortem diagnostics include identification of intranuclear inclusion bodies in the liver, PCR assay, and/or FISH of infected tissue.


Sample type:

    Product Advantage:

    • High Detection accuracy
    • High cost performance
    • Quality assurance
    • Fast delivery

    Intended Use

    HG Rapid Test ICH is a chromatographic immunoassay kit for rapid and qualitative detection of canine adenovirus 1(CAV1) antigens in blood,which cause infectious canine hepatitis (ICH). The CAV1-specificantibody is immobilized onto nitrocellulose membrane. And also,another CAV1-specific antibodies are conjugated to 40 nm of colloidal gold particles. This conjugate is placed on a polyester or glass pad as conjugate pad. When the sample is dropped into the sample well on the device, the solubilized conjugate migrates with the sample by passive diffusion and both the conjugate and sample come into contact with the antibodies that are immobilized onto the nitrocellulose. If the sample contains adenovirus antigens, the result is visible within 10 minutes in the form of a red line that develops on the membrane. The solution continues to migrate to encounter a control reagent, thereby producing a red line (control line).

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    Symptons

    1.Fever,

    2.Lethargy,

    3.Inappetence,

    4.Vomiting,

    5.Hemorrhagic diarrhea,

    6.Abdominal pain,

    7.Dehydration,

    8.Conjunctivitis,

    9.Petechial hemorrhages,

    10.Tachypnea,

    Features

    1 This product uses the principle of colloidal gold immunochromatography to quickly detect ICH Antigen.

    2 This product does not require special instruments, and can be quickly and easily implemented on-site inspection.

    3 Using high-purity raw materials and high-quality materials, it shows extremely high sensitivity and specificity.

    Operation

    Place all specimens, test devices, and Assay solution and allow them to room temperature prior to testing (15~30 min).

    Prepare the test device as you need,and then mark the patients,ID onto the device,Please perform the test immediately after removing the device from foil pouch.

    Load 1 drop (10ul) of specimen into the sample well (S) with capillary tube.The sample volume to indicator line 10 μL.

    Add 3 drops of Assay Solution into the sample well (S).

    lnterpret the testing result within 10 minutes. Do not read the result after 10 minutes.

    ICH test

    Interpretation of Results

    Interpretation of Results

    This product is aimed to facilitate public health institute worldwide to increase their diagnostic capabilities when facing the emerging Infectious Canine Hepatitis virus health challenge, allowing for an efficient and rapid public health response in the case of an outbreak.

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